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Journal: Journal of Oral Microbiology
Article Title: Diagnostic oral microbiota signatures for gastric cancer and associations with carcinogenic signaling pathways
doi: 10.1080/20002297.2026.2613531
Figure Lengend Snippet: Expression of apoptosis-related genes in GC and HCs. (A) Heatmap of the Spearman’s rank correlation analysis between 8 genera and 30 significant pathways in GC. (B) Heatmap of the Spearman’s rank correlation analysis between 8 genera and 63 significant orthologs in GC. (C) Correlation analysis between 3 genera (columns) and 4 functional profiles (Ko04215-apoptosis, K07190-phosphorylase kinase alpha/beta subunit, K12767-serine/threonine-protein kinase, K08291-G protein-coupled receptor kinase) in GC and HCs. Full pathway and ortholog names are provided in Table S8. (D–I) Plasma level of (D) IL-6, (E) STAT3, (F) TGF- β , (G) NF-kB, (H) p -Smad2, and (I) p -Smad3 from 16 samples selected based on age and sex variance in each group. (J) Correlation analysis between 3 genera (columns) and 6 target molecules (rows) in GC and HCs. * p < 0.05, ** p < 0.01, and *** p < 0.001. PKA; phosphorylase kinase alpha/beta subunit, AKT; protein kinase B, GRK; G protein-coupled receptor kinases, IL-6; Interleukin-6, STAT3; Signal transducer and activator of transcription 3, TGF-β1; Transforming growth factor beta 1, NF-κB; Nuclear factor kappa-light-chain-enhancer of activated B cells. All p -values were adjusted for multiple comparisons using the Benjamini–Hochberg FDR correction.
Article Snippet: Kits for the growth differentiation factor 15 (#BMS2258) and transforming growth factor beta 1 (TGF-β1) (#BMS249-4) were purchased from Life Technologies; those for the nuclear factor kappa-light-chain-enhancer of activated
Techniques: Expressing, Functional Assay, Clinical Proteomics
Journal: Journal of Oral Microbiology
Article Title: Diagnostic oral microbiota signatures for gastric cancer and associations with carcinogenic signaling pathways
doi: 10.1080/20002297.2026.2613531
Figure Lengend Snippet: Proposed mechanism of gastric carcinogenesis illustrating the directionality of molecular differences observed in this study. The schematic framework is adapted from previously reported carcinogenic signalling pathways in gastric cancer, including the TGF-β/Smad2/3 pathway [ , ], the PI3K/AKT/NF-κB pathway , and the IL-6–mediated JAK/STAT3 pathway [ , ]. Molecules analysed in this study are highlighted, with red and blue arrows indicating relatively higher and lower levels, respectively, in patients with GC compared with HCs, based on group-wise comparisons of PICRUSt-inferred orthologs and plasma cytokine measurements. The figure is intended to contextualise these observational findings within established literature-based signalling frameworks and does not represent direct experimental validation of mechanistic pathways. TGF-β; transforming growth factor beta, TFGBR; TGF- β receptor, PI3K; phosphoinositide 3-kinases, AKT; protein kinase B, GPCR; G protein-coupled receptor, GRK; G protein-coupled receptor kinases, NF-κB; nuclear factor kappa-light-chain-enhancer of activated B cells, IL-6; interleukin 6, STAT3; signal transducer and activator of transcription 3, JAK; Janus kinase, PTEN; phosphatase and tensin homologue.
Article Snippet: Kits for the growth differentiation factor 15 (#BMS2258) and transforming growth factor beta 1 (TGF-β1) (#BMS249-4) were purchased from Life Technologies; those for the nuclear factor kappa-light-chain-enhancer of activated
Techniques: Clinical Proteomics, Biomarker Discovery